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Benzo[a]pyrene (B[a]p) is an environmental pollutant that has been implicated in carcinogenesis. Sacchammyces cerevisiae was treated with B[a]P, and the responses of its cytochrome P450 (CYP) enzyme and DNA- damage checkpoint genes were examined through gene expression profiles using a reverse transcription polymerase chain reaction (RT-PCR). The DNA-damage checkpoint genes tested were the chkl and pds1 genes, involved in a metaphase arrest, the swih gene targeted by GI arrest, the po12 gene related to S phase arrest. and the cln2 gene encoding a cyclin protein. all of which are based on rad9 and rad24. Among these genes, no noticeable effect was found when the cells were exposed to various concentrations of B[a]P. However, the transcriptional activity of CYP5I was significantly different when the cells were exposed to B[a]P. Accordingly, the present results indicate that cytochrome P4SO play.; a more significant role than DNA-damage checkpoint genes in the response of S. cerevisiae to B[a]P.
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